Additional file 3.

File showing that expression of putative stem cell markers (CD44+/CD24- and CD133+) occurs on distinct and non-overlapping cell populations. (A) A1.8 cells stained simultaneously with antibodies for CD44, CD24, and CD133 (upper panel). The lower panel shows compensated dual staining for CD44/CD133, CD44/CD24, and triple staining for all three markers. Only 0.02% of A1.8 cells express all 3 markers. (B) RP.1 cells were stained and analyzed as above. No cells bearing all three markers are detectable. One of two independent analyses is shown here.

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Wright et al. Breast Cancer Research 2008 10:R10   doi:10.1186/bcr1855