Additional file 4.

File showing that RP.1 cells growing as spheroids in the absence of attachment are enriched in CD133⁺ cells. (A) Parental cells and (B) cells dissociated from spheroids after expanding for four passages in vitro were stained side-by-side for CD133 and examined by fluorescence-activated cell sorting. The percentage of CD133⁺ cells is indicated in each box. Note that a distinct CD133^High population is now evident in spheroid-derived cells. One of three independent experiments is shown here.

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Wright et al. Breast Cancer Research 2008 10:R10   doi:10.1186/bcr1855