Additional file 4.
File showing that RP.1 cells growing as spheroids in the absence of attachment are enriched in CD133+ cells. (A) Parental cells and (B) cells dissociated from spheroids after expanding for four passages in vitro were stained side-by-side for CD133 and examined by fluorescence-activated cell sorting. The percentage of CD133+ cells is indicated in each box. Note that a distinct CD133High population is now evident in spheroid-derived cells. One of three independent experiments is shown here.
Format: PPT Size: 137KB Download file
This file can be viewed with: Microsoft PowerPoint Viewer
Wright et al. Breast Cancer Research 2008 10:R10 doi:10.1186/bcr1855