Table 1

Expression of stem cell genes in cells that express stem cell markers

Gene

A1.8: SC+/SC-

RP1: CD133+/CD133-


T

>20

4.6

Sox1

19.2

2.2

Aldh1a1

3.5

3.2

Fgfr1

3.2

2.1

Tert

1.2

4.4

Notch1

2.5

4.1

CD44

1.9

3.4

Ascl2

7.2

Pdx1

7.1

Acan

6.6

Dhh

4.3

ALDH2

3

MME

4.5

KRT15

17

JAG1

2.2

FOXa1

19

DLL1

6

DLL3

3

COL9a1

3

CDH1

11

CD8B

6

CCNA2

2.5


Expression of genes from a panel of 84 stem cell-associated genes was determined by SuperArray real-time PCR using RNA from A1.8 and RP.1 cells sorted for CD44, CD24, or CD133 markers (SC+ and SC-). For a complete list of genes tested, see [48]. The results are expressed as fold increase in cells sorted positive over negative markers from replicate experiments. Data are normalized to five housekeeping genes, as per the manufacturers' instructions. Genes common for both cell lines are presented at the top.

Wright et al. Breast Cancer Research 2008 10:R10   doi:10.1186/bcr1855

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