Figure 4.

Methylation analysis of BRCA1 in KCF1 and KCF4. (a) MethyLight results for BRCA1 and the control gene HMBS for samples from KCF1 and KCF4. The BRCA1 MethyLight assay indicates the presence of methylated DNA for the peripheral blood (PBL) and tumour (TUM) for both individuals. (b) The methylation-sensitive high resolution melting (MS-HRM) assay also shows the presence of methylation in the peripheral blood and tumour for both individuals.