Figure 9.

Pulse exposures of cancer cells to ibandronate in presence of low or high calcium concentrations. MDA-MB-231 and MCF-7 cells were incubated for 1, 6, or 72 hours with ibandronate (1 to 1,000 μmol/l) in culture medium containing 0.6 mmol/l calcium (filled circles) or 1.6 mmol/l calcium (open circles). Cell culture densities were evaluated at 72 hours by crystal violet staining assay. Data are presented as percentages (mean ± standard deviation) of control values, which refer to cultures with 0.6 mmol/l calcium and without ibandronate. Mean of results pooled from three experiments (n = 18).