Figure 3.

NHERF1 accelerates decay of p-Akt induced by PDGF. (a) MCF10A cells over-expressing Na⁺/H⁺ exchanger regulatory factor 1 (NHERF1) or control vector (Babe) were serum starved and restimulated with platelet-derived growth factor for 0 to 120 minutes as indicated. Cells were lysed for measurement of phospho-Akt (p-Akt) level by immunoblotting. Expression status of NHERF1 was verified. Total Akt was also determined to normalize loading. (b) Zr75.1 cells infected with NHERF1 knockdown vector (910) or control (Babe) were analyzed as described for panel a.