Breast Cancer Research
Volume 10
Issue 2

Viewing options:

Abstract
Full text
PDF (2.6MB)
Additional files

Associated material:

PubMed record

Related literature:

Articles citing this article
on Google Scholar
on ISI Web of Science
on PubMed Central
Other articles by authors
on Google Scholar

Fillmore CM
Kuperwasser C

on PubMed

Fillmore CM
Kuperwasser C
Related articles/pages
on Google

on Google Scholar

on PubMed

Tools:

Post to:

Research article

Human breast cancer cell lines contain stem-like cells that self-renew, give rise to phenotypically diverse progeny and survive chemotherapy

Christine M Fillmore¹ and Charlotte Kuperwasser²^,3

¹Department of Genetics, Sackler School, Tufts University School of Medicine, Harrison Avenue, Boston, Massachusetts 02111, USA

²Department of Anatomy & Cellular Biology, Sackler School, Tufts University School of Medicine, Harrison Avenue, Boston, Massachusetts 02111, USA

³Department of Radiation Oncology, Tufts-New England Medical Center, Molecular Oncology Research Institute, Boston, Massachusetts 02111, USA

author email corresponding author email

Breast Cancer Research 2008, 10:R25doi:10.1186/bcr1982


Published:	26 March 2008

Additional files

Additional file 1:

File containing a table that provides the characteristics of cell lines in vivo.

Format: DOC Size: 21KB Download file

This file can be viewed with: Microsoft Word Viewer

Additional file 2:

File showing representative flow cytometry profiles of CD44 and CD24 expression in human breast cell lines.

Format: PDF Size: 70KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 4:

(A) SUM1315 and SUM149 adherent cultures and tumorspheres smaller than 100 μm analyzed by flow cytometry for CD44⁺/CD24^-/ESA⁺cells. (B) SUM159 cells were plated in parallel and grown for 2 weeks in adherent or nonadherent cultures. At the indicated times, cells were harvested and counted. (C) Crystal violet growth curves of adherent cells from various cell lines. (D) Flow cytometry histograms of SUM159 and MDA.MB.231 cells pulsed with BrdU for 10 days. Note that nearly 100% of the cells are labeled.

Format: PDF Size: 47KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 5:

(A) Flow cytometry histograms of SUM159 and MDA.MB.231 cells pulsed with BrdU for 10 days. Note that nearly 100% of the cells are labeled. (B) Proliferating cultures of SUM159 cells were pulse-chased and analyzed by flow cytometry for BrdU CD44. Note that the cells containing the highest 1% BrdU label are only 30% CD44⁺/CD24^-/ESA⁺cells.

Format: PDF Size: 34KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 6:

(A) Representative phase-contrast brightfield images of SUM159 cultures after 6 days of exposure to indicated doses of chemotherapy. (B) Quantification of the average percent killing by 10 nmol/l Taxol or 1 mmol/l 5-FU 6 day treatments for SUM159, SUM149, and MDA.MB.231 cell lines. As shown, the percentage reflects both the number of cells that remain adherent post-treatment relative to a placebo treated control plate as determined by hemacytometer counting, and the percentage of adherent cells that do not take up PI stain as determined by flow cytometry. Error bars indicate mean ± standard error of the mean. (C) Immunofluorescence of SUM159 cells stained for BrdU (green) and DAPI (4',6 diamidino-2-phenylindole; blue) at 6 days after chase with 10 nmol/l Taxol or 1 mmol/l 5-FU.

Format: PDF Size: 153KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 3:

(A) Additional representative flow cytometry analysis sorting scheme for in vivo injections. SUM159 cells were sorted on ESA. Dot plots show resulting CD44⁺/CD24^-/low/ESA⁺or CD44⁺/CD24^-/ESA^-fractions. (B) SUM149 cells were sorted on ESA. Dot plots show resulting CD44⁺/CD24^-/low/ESA⁺or CD44⁺/CD24⁺/ESA⁺fractions.

Format: PDF Size: 53KB Download file

This file can be viewed with: Adobe Acrobat Reader