|
Primer sequences for MSP |
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| Gene |
Methylated/unmethylated |
Direction |
Sequence |
|
|
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| RASSF1A |
Methylated |
Forward |
5'-GTGTTAACGCGTTGCGTATC-3' |
| Reverse |
5'-AACCCCGCGAACTAAAAACGA-3' |
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| Unmethylated |
Forward |
5'-TTTGGTTGGAGTGTGTTAATGTG-3' |
|
| Reverse |
5'-CAAACCCCACAAACTAAAAACAA-3' |
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| CCND2 |
Methylated |
Forward |
5'-TACGTGTTAGGGTCGATCG-3' |
| Reverse |
5'-CGAAAACATAAAACCTCCACG-3' |
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| Unmethylated |
Forward |
5'-GTTATGTTATGTTTGTTGTATG-3' |
|
| Reverse |
5'-TAAAATCCACCAACACAATCA-3' |
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| GSTP1 |
Methylated |
Forward |
5'-TTCGGGGTGTAGCGGTCGTC-3' |
| Reverse |
5'-GCCCCAATACTAAATCACGACG-3' |
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| Unmethylated |
Forward |
5'-GATGTTTGGGGTGTAGTGGTTGTT-3' |
|
| Reverse |
5'-CCACCCCAATACTAAATCACAACA-3' |
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| APC |
Methylated |
Forward |
5'-TATTGCGGAGTGCGGGTC-3' |
| Reverse |
5'-TCGACGAACTCCCGACGA-3' |
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| Unmethylated |
Forward |
5'-GTGTTTTATTGTGGAGTGTGGGTT-3' |
|
| Reverse |
5'-CCAATCACAAACTCCCAACAA-3' |
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| TWIST |
Methylated |
Forward |
5'-TTTCGGATGGGGTTGTTATCG-3' |
| Reverse |
5'-GACGAACGCGAAACGATTTC-3' |
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| Unmethylated |
Forward |
5'-TTGGATGGGGTTGTTATTGT-3' |
|
| Reverse |
5'-ACCTTCCTCCAACAAACACA-3' |
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| NES1 |
Methylated |
Forward |
5'-TTCGAAGTTTATGGCGTTTC-3' |
| Reverse |
5'-TTATTTCCGCAATACGCGAC-3' |
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| Unmethylated |
Forward |
5'-TTGTAGAGGTGGTGTTGTTT-3' |
|
| Reverse |
5'-CACACAATAAAACAAAAAACCA-3' |
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| RARβ2 |
Methylated |
Forward |
5'-GAACGCGAGCGATTCGAGT-3' |
| Reverse |
5'-GACCAATCCAACCGAAACG-3' |
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| Unmethylated |
Forward |
5'-GGATTGGGATGTTGAGAATGT-3' |
|
| Reverse |
5'-CAACCAATCCAACCAAAACAA-3' |
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| CDH1 |
Methylated |
Forward |
5'-TTAGGTTAGAGGGTTATCGCGT-3' |
| Reverse |
5'-TAACTAAAAATTCACCTACCGAC-3' |
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| Unmethylated |
Forward |
5'-TAATTTTAGGTTAGAGGGTTATTGT-3' |
|
| Reverse |
5'-CACAACCAATCAACAAC ACA-3' |
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|
MSP, methylation-specific PCR. | |||
Sunami et al. Breast Cancer Research 2008 10:R46 doi:10.1186/bcr2098 |
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