Additional file 3.

A tif file showing serial transplantation of MINOSphere-derived outgrowth. Histology (hematoxylin and eosin stained, formalin-fixed, paraffin-embedded 4 μm sections) of donor (A, D), second (B, E), and third (C, F) serial transplantations from the single cell MINOsphere derived outgrowths from MINO line D (panels A, B, and C) and MINO line 4 (panels D, E, and F). Line D (panels A, B, and C) all show a microacinar pattern and transplant attempts resulted in growth of 75% (n = 8) of second serial and 100% (n = 16) of third serial transplant attempts. MINO line 4 (panels d, e, and f) shows a solid lobulated pattern typical of line 4 and transplant attempts resulted in growth of 67% (n = 6) and 100% (n = 8) of third serial transplants. All panels are identical magnification, with the 50 μm scale bar shown (panel f).

Format: TIFF Size: 5.6MB Download file

Damonte et al. Breast Cancer Research 2008 10:R50   doi:10.1186/bcr2104