Research article
Genes responsive to both oxidant stress and loss of estrogen receptor function identify a poor prognosis group of estrogen receptor positive primary breast cancers
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* Corresponding author: Christopher C Benz cbenz@buckinstitute.org
Buck Institute for Age Research, Redwood Boulevard, Novato, California 94945, USA
Breast Cancer Research 2008, 10:R61 doi:10.1186/bcr2120
See related editorial by Neven et al., http://breast-cancer-research.com/content/10/5/109
Published: 17 July 2008Additional files
Additional file 1:
A PDF file showing oxidant treatment effects on cell viability, ER content, and ER transcriptional activity. Methods for the measurement of cell viability, ER content, and transcriptional activation activity at the selected concentration of each oxidant (diamide, H2O2, and menadione [K3]) are detailed, with pertaining results shown in Figures S1 to S3.
Format: PDF Size: 866KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 2:
An Excel file showing gene sets used for GSEA. All gene sets were mapped to corresponding Unigene symbols for input into GSEA software.
Format: XLS Size: 74KB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file 3:
An Excel file providing a list of genes responsive to each of the eight different MCF7 treatment conditions (72 hours of estrogen [E] deprivation; 72 hours of ER-α siRNA knockdown; 8 hours of diamide; 8 hours H2O2 and 8 hours of menadione [K3]; 72 hours of ER-α siRNA and 8 hours diamide; 72 hours ER-α siRNA and 8 hours of H2O2; 72 hours of ER-α siRNA and 8 hours K3).
Format: XLS Size: 695KB Download file
This file can be viewed with: Microsoft Excel Viewer