Figure 2.

Synthetic average composite gels showing spots matched across all eight IEF gels (24 SDS-PAGE gels). Images were generated using the GREG software and labels were added manually. The average ER-positive signal is indicated as blue, the average ER-negative signal is indicated as orange, and equal intensities of both signals give gray or black pixels. Spot numbers correspond to Figure 3, whereby spots from pH4-5 gel are designated with spot numbers 1-nn, spots from pH5-6 gel are designated with spot numbers 2-nn, and spots from pH6-9 gel are designated with spot numbers 3-nn. For example, PGRMC1 spot 1–24 from Figure 3 is the left-most spot labeled in the pH4-5 gel. Colored signals without spot labels were either unidentifiable or were not consistently differential, with average intensities being markedly influenced by one or few samples and thereby failing to achieve statistical differential significance across the dataset. ER, estrogen receptor; IEF, isoelectric focusing; PGMRC, progesterone receptor membrane component.