Research article
Anti-oestrogens but not oestrogen deprivation promote cellular invasion in intercellular adhesion-deficient breast cancer cells
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* Corresponding author: Stephen Hiscox Hiscoxse1@cf.ac.uk
1 Velindre Cancer Centre, Velindre Road, Cardiff, CF14 2TL, UK
2 Tenovus Centre for Cancer Research, Welsh School of Pharmacy, Cardiff University, King Edward VII Avenue, Cardiff, CF10 3NB, UK
Breast Cancer Research 2008, 10:R103 doi:10.1186/bcr2206
Published: 4 December 2008Additional files
Additional file 1:
Figure that demonstrates the ability of tamoxifen to promote the invasion, and an increase in Src activation, of an additional ER+ cell line, T47D, following E-cadherin knockdown. T47D cells were treated with non-targeting siRNA control (NT control) or siRNA for E-cadherin (CDH1) in the absence of oestrogen (-E2) or presence of tamoxifen (tam) as described for MCF-7 cells. (a) Cell invasion was assessed using Matrigel invasion assays while changes in E-cadherin expression and Src activity were determined by (b) Western blotting (c) with subsequent densitometry. Tamoxifen promoted significant cell invasion in the absence of E-cadherin expression which was accompanied by an increase in Src kinase activity (Src phosphorylated at Y418).
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