Anti-oestrogens but not oestrogen deprivation promote cellular invasion in intercellular adhesion-deficient breast cancer cells

Additional file 1:

Figure that demonstrates the ability of tamoxifen to promote the invasion, and an increase in Src activation, of an additional ER+ cell line, T47D, following E-cadherin knockdown. T47D cells were treated with non-targeting siRNA control (NT control) or siRNA for E-cadherin (CDH1) in the absence of oestrogen (-E2) or presence of tamoxifen (tam) as described for MCF-7 cells. (a) Cell invasion was assessed using Matrigel invasion assays while changes in E-cadherin expression and Src activity were determined by (b) Western blotting (c) with subsequent densitometry. Tamoxifen promoted significant cell invasion in the absence of E-cadherin expression which was accompanied by an increase in Src kinase activity (Src phosphorylated at Y418).

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