Research article
Buthionine sulfoximine sensitizes antihormone-resistant human breast cancer cells to estrogen-induced apoptosis
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* Corresponding author: V Craig Jordan v.craig.jordan@fccc.edu
1 Department of Medical Sciences, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA, 19111, USA
2 Department of Surgical Oncology, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA, 19111, USA
3 Department of Radiation Oncology, University of Pennsylvania, 195 John Morgan Building, 3620 Hamilton Walk, Philadelphia, PA 19104, USA
4 Department of Pathology, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA, 19111, USA
Breast Cancer Research 2008, 10:R104 doi:10.1186/bcr2208
Published: 5 December 2008Additional files
Additional file 1:
Powerpoint file showing the growth inhibitory effect of buthionine sulfoximine (BSO) and 17β-estradiol (E2) in MCF-7:2A cells is reversed by the antiestrogen 4-hydroxytamoxifen (4-OHT). MCF-7:2A cells (30,000/well) were seeded in 24-well plates and after 24 h were treated with < 0.1% ethanol vehicle (control), 1 nM E2, 100 μM BSO, 100 μM BSO plus 1 nM E2, 1 μM 4-OHT, 4-OHT + E2, 4-OHT + BSO, 4-OHT + E2 + BSO for 7 days. At the indicated time points, cells were harvested and total DNA (μg/well) was quantitated as described in Materials and methods. The data represent the mean of three independent experiments; bars, ± standard error of the mean (SEM). *, p < 0.01 compared with control cells; #, p < 0.01 compared to E2-treated cells.
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