Table 2 |
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|
Reverse library – Genes upregulation in the absence of prolactin |
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|
Confirmed |
Observed |
Function |
Accession number |
Protein name |
|
|
||||
|
RNA-related functions |
||||
|
|
||||
|
Splicing factor |
[Swiss-Prot:O75533] |
Splicing factor 3B subunit 1, spliceosome-associated protein 155 |
||
|
Translational regulation |
[Swiss-Prot:Q8TB72] |
Pumilio homolog 2, pumilio-2, KIAA0235 fragment |
||
|
|
||||
|
Chaperone |
||||
|
|
||||
|
Chaperone |
[Swiss-Prot:O60925] |
Prefoldin subunit 1 |
||
|
|
||||
|
Protein synthesis |
||||
|
|
||||
|
* |
Activates the trk oncogene |
[Swiss-Prot:P62424] |
60S ribosomal protein L7A, surfeit locus protein 3, PLA-X polypeptide |
|
|
|
||||
|
Electron transport |
||||
|
|
||||
|
Electron transport |
[GenBank:AAC25442] |
NADH dehydrogenase subunit 2 homo sapiens |
||
|
Selenium metabolism and protection oxidative stress |
[Swiss-Prot:Q99475] |
Km-102-derived reductase like factor, thioredoxin reductase |
||
|
Electron transport |
[RefSeq:NP_055217] + [GenBank:EAW62308] [GenBank:AAH90048] [GenBank:AAH01390] [Swiss-Prot:O14949] |
Ubiquinol-cytochrome c reductase complex ubiquinone binding protein QP-C, complex III subunit VII |
||
|
|
||||
|
Transport |
||||
|
|
||||
|
* |
Vesicle trafficking protein transport |
[GenBank:BAA07558] |
Hypothetical protein KIAA0079, HA3543, SEC24-related protein C |
|
|
* |
Protein transport |
[GenBank:CAI15005] |
Coatomer alpha subunit, Alpha-COP |
|
|
|
||||
|
Proteasome |
||||
|
|
||||
|
* |
Proteasome |
[Swiss-Prot:P25788] |
Proteasome component C8, macropain subunit C8, proteasome subunit alpha type 3 multicatalytic endopeptidase complex subunit C8 |
|
|
|
||||
|
Ubiquitination |
||||
|
|
||||
|
Deubiquitinating enzyme tumour suppressor |
[GenBank:BAA74872] |
KIAA0849 protein, CYLD gene |
||
|
Ubiquitin-specific protease cysteine proteases |
[GenBank:AAH64516] [GenBank:NP_005144] [Swiss-Prot:Q14694] [GenBank:ABM86690] [GenBank:ABM83479] |
KIAA0190 ubiquitin specific peptidase 10 |
||
|
* |
Ubiquitin cycle |
[GI:4929720] [GenBank:NP_057490] [Swiss-Prot:Q9Y3C8] [GenBank:AF151884] |
Ubiquitin-fold modifier conjugating enzyme 1 |
|
|
|
||||
|
Transcription factors |
||||
|
|
||||
|
* |
Transcription factor |
[Swiss-Prot:Q12905] |
Interleukin enhancer-binding factor 2, NF45 protein |
|
|
Transcription factor complex, RNA binding |
[GenBank:CAA10029] |
NS1-binding protein |
||
|
|
||||
|
Miscellaneous |
||||
|
|
||||
|
* |
2 |
N-oligosaccharyl transferase complex |
[GenBank:CAB41763] |
DJ343K2.2.1, ribophorin II isoform 1 |
|
Unknown |
[Swiss-Prot:O95801] |
Tetratricopeptide repeat protein 4 |
||
|
Kinase |
[GenBank:BAA76815] |
KIAA0971 protein, FAST kinase domains 2 |
||
|
Mitochondrial fusion |
[Swiss-Prot:O95140] [GenBank:AAD02058] |
MFN2, KIAA0214 protein, CPRP1 |
||
|
Calcium binding |
[Swiss-Prot:P62158] |
Calmodulin |
||
|
Glycolytic and gluconeogenesis pathways Second product-transcription factor |
[Swiss-Prot:P06733] |
Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, enolase 1, MBP-1, plasminogen-binding protein |
||
|
2 |
Palmitoyl-(protein) hydrolase activity |
[PIR:I58097] |
Palmitoyl protein thioesterase precursor, EC 3.1.2.22 |
|
|
* |
Iron ion transport |
[GenBank:gi:37432] |
Transferrin receptor, p90, CD71 |
|
|
* |
2 |
Unknown OR Wnt signalling pathway |
[GenBank:gi:1167502] [GenBank:gi:1524104] |
Hypothetical protein TI-227H wnt 13 |
|
|
||||
|
Prolactin-regulated genes. cDNA fragments identified preferentially in the reverse libraries (absence of prolactin) were sequenced. Sequences that were identical to those that encoded human proteins are presented using the protein accession number. Genes that were identified more than once are indicated as the number of times observed. Genes that were confirmed by a second screen are marked with an asterisk. Alpha-COP = alpha coat protein; CPRP1 = caprine prolactin-related protein-1; CYLD = cylindromatosis; FAST = Fas-activated serine/threonine; MFN2 = mitofusin-2, transmembrane GTPase; MBP-1 = C-myc promoter-binding protein; NADH = nicotinamide adenine dinucleotide plus hydrogen; NS1 = non-structural 1 protein; NF45 = nuclear factor of activated T-cells 45 kDa; wnt 13 = wingless-type MMTV (mouse mammary tumour virus) integration site family, member 2B. |
||||
|
Perotti et al. Breast Cancer Research 2008 10:R94 doi:10.1186/bcr2193 |
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