Figure 5.

Apoptosis in TEBs. H&E stained TEBs in +/+ transplanted glands (top left) have few apoptotic nuclei and one cleaved caspase 3 positive (brown stain) cell (middle left). H&E stained TEBs in -/- transplanted glands (top right) have numerous apoptotic nuclei and cleaved caspase 3 positive cells (middle right). BrdU-positive cells (brown stain) are present in both the +/+ transplanted glands (bottom left) and in the -/- transplanted glands (bottom right). BrdU, 5-bromo-2-deoxyuridine; cc3, cleaved caspase 3; H&E, hematoxylin and eosin; TEB, terminal end bud.