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Open Access Highly Access Research article

Pregnancy in the mature adult mouse does not alter the proportion of mammary epithelial stem/progenitor cells

Kara L Britt1,2, Howard Kendrick1, Joseph L Regan1, Gemma Molyneux1, Fiona-Ann Magnay1, Alan Ashworth1 and Matthew J Smalley1*

Author Affiliations

1 Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK

2 Monash Institute of Medical Research, Monash Medical Centre, Clayton Road, Clayton 3168, Australia

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Breast Cancer Research 2009, 11:R20 doi:10.1186/bcr2245

Published: 23 April 2009

Additional files

Additional file 1:

An Adobe Photoshop TIF file containing an image that shows gating mammary epithelial cells for CD49 expression, the data from the CD24 CD49f flow cytometry plots in Figure 2 plotted as 5% linear density contour plots to delineate the main body of CD24+/Low cells. The CD24+/Low CD49fHigh gate is set at the edge of the main body of cells.

Format: TIFF Size: 725KB Download file

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Additional file 2:

An Adobe Photoshop TIF file containing an image that shows a comparison of normalised mean forward scatter/side scatter values for CD24+/Low Sca-1-, CD24+/High Sca-1- and CD24+/High Sca-1+ mammary epithelial cells. The figure shows mean forward scatter (left-hand column) and side scatter (right-hand column) values normalised to lymphocyte forward scatter and side scatter values for the CD24+/Low Sca-1- (basal), CD24+/High Sca-1- (luminal ER-) and CD24+/High Sca-1+ (luminal ER+) populations isolated from AMV (top row) and parous (bottom row) mammary tissue (n = 3 independent sorts).

Format: TIFF Size: 403KB Download file

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Additional file 3:

An Adobe Photoshop TIF file containing an image that shows changes in normalised mean forward scatter/side scatter values of mammary epithelial cell subpopulations between parous and AMV mammary tissue. The figure shows mean forward scatter and side scatter values normalised to lymphocyte forward scatter and side scatter values in AMV and parous tissue for the CD24+/Low Sca-1- (basal; top), CD24+/High Sca-1- (luminal ER-; middle) and CD24+/High Sca-1+ (luminal ER+; bottom) populations (n = 3 independent sorts).

Format: TIFF Size: 419KB Download file

Open Data