Additional data file 1:

Adobe file containing a figure showing proliferation suppression of MDA-MB-231 cells by sFRP1 CM treatment. One thousand MDA-MB-231 cells were seeded per well of a 96-well plate and proliferation was measured with a YOPRO assay after 3 days of treatment with sFRP1 CM or control CM.

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Additional data file 2:

Adobe file containing a figure showing additional xenograft experiments with MDA-MB-231/sFRP1-P1 and control P1 cells. (a), (b) The onset of tumor appearance in two independent xenograft experiments with MDA-MB-231/sFRP1-P1 and control-P1 cells: (a) six mice per group, P: 0.0179 (log-rank test); (b) eight mice per group, P: 0.0223 (log-rank test).

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Additional data file 3:

Adobe file containing a figure showing known WNT target genes whose expression was downregulated upon sFRP1 expression both in vitro and in vivo. Normalized microarray results of established WNT pathway target genes listed in Additional data file 4.

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Additional data file 4:

Word file containing a table that lists established WNT pathway target genes whose expression was suppressed upon sFRP1 expression both in vitro and in vivo. Expression of established WNT pathway target genes was examined by microarray analysis. Listed here are genes whose expression was suppressed upon sFRP1 expression both in vitro and in vivo.

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Additional data file 5:

Word file containing a table that lists genes whose expression was downregulated upon sFRP1 expression only in vivo. List of 106 identified genes shown in Figure 6a. Fold-change refers to expression changes between sFRP1-positive and control tumors. For some genes there are multiple probesets with different values of fold-change.

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Additional data file 6:

Word file containing a table that lists genes whose expression was upregulated upon sFRP1 expression only in vivo. List of 62 identified genes shown in Figure 6b. Fold-change refers to expression changes between sFRP1-positive and control tumors. For some genes there are multiple probesets with different values of fold-change.

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Additional data file 7:

Adobe file containing a figure showing c-Myc expression in MDA-MB-231/sFRP1-P1 and control-P1 tumors. (a) Quantitative real-time PCR analysis for c-Myc RNA in tumor lysates from six sFRP1-positive tumors and five control tumors. Average ± standard error of the mean. n.s.: not significant. (b) Lysates from six MDA-MB-231/sFRP1-P1 tumors and two control-P1 tumors were examined for c-Myc and Myc-tagged sFRP1 with a c-Myc antiserum; α-tubulin serves as a loading control.

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Additional data file 8:

Adobe file containing a containing a figure showing in vitro and in vivo gene expression of β₁-integrin and extracellular matrix components. Microarray results of the genes coding β₁-integrin and some extracellular matrix components.

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Additional data file 9:

Adobe file containing a figure showing fluorescence-activated cell sorting analysis for active β₁-integrin. MDA-MB-231/sFRP1-P1 and control-P1 cells were incubated with an antibody recognizing active β₁-integrin, followed by incubation with FITC conjugated secondary antibody. Fluorescence was measured using a FACSCalibur machine and the percentage of gated cells stained with active β₁-integrin was calculated.

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