Additional data file 9.Adobe file containing a figure showing fluorescence-activated cell sorting analysis for active β1-integrin. MDA-MB-231/sFRP1-P1 and control-P1 cells were incubated with an antibody recognizing active β1-integrin, followed by incubation with FITC conjugated secondary antibody. Fluorescence was measured using a FACSCalibur machine and the percentage of gated cells stained with active β1-integrin was calculated. Format: EPS Size: 375KB Download file Matsuda et al. Breast Cancer Research 2009 11:R32 doi:10.1186/bcr2317 |