Research article
Targeting inhibitor of apoptosis proteins in combination with ErbB antagonists in breast cancer
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* Corresponding author: Charles H Streuli charles.streuli@manchester.ac.uk
1 Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK
2 Faculty of Medical and Human Sciences, University of Manchester, Manchester M20 4BX, UK
3 Department of Surgery, South Manchester University Hospitals Trust, Wythenshawe Hospital, Southmoor Road, Manchester M23 9LT, UK
Breast Cancer Research 2009, 11:R41 doi:10.1186/bcr2328
Published: 29 June 2009Additional files
Additional file 1:
Adobe file containing a figure that shows cIAP levels in breast cancer cell lines. Relative migration positions of cIAP1 and cIAP2 were determined on the Li-Cor Odyssey™ system prior to samples being re-run and probed with enhanced chemiluminescence. A long exposure is shown, where MDAMB468, Sum225, Sum190 and Zr-75-1 cell lines show detectable cIAP2 levels, although these levels are still lower than that observed in the nonmalignant MCF10a cell line.
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Additional file 2:
Adobe file containing a figure that shows the effect of a second XIAP siRNA and the scrambled (mock) siRNA oligonucleotides on TRAIL (10 ng/ml) or Gefitinib (10 μM)-induced apoptosis in BT474 cells. Data are presented as fold changes in apoptosis (mean ± standard error of the mean). nt: nontreated.
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Additional file 3:
Adobe file containing a figure that shows the effect of Smac mimetic on TRAIL-induced apoptosis in MCF10a cells. MCF10a cells were pretreated with the Smac mimetic for 2 hours prior to TRAIL (10 ng/ml) addition for 48 hours. Data are presented as fold changes in apoptosis (mean ± standard error of the mean). nt: nontreated; con: no Smac mimetic.
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Additional file 4:
Adobe file containing a table that lists prognostic information on tumour samples. Steroid receptor (oestrogen receptor percentage/progesterone receptor percentage), proliferative index (%Ki67), and growth factor receptor status (EGFR, C-erbB-2) as well as tumour type, grade and lymph node metastasis (number positive/number examined) were determined in the clinic.
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Additional file 5:
Adobe file containing a figure that shows the effect of Survivin knockdown on ErbB antagonist or TRAIL-induced apoptosis in BT474 cells. Data presented as mean ± standard error of the mean.
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