Table 1

Limiting dilution analysis of wild-type or Csf1^op/op CD29^hiCD24⁺ cells transplanted into cleared mammary fat pads

Number of CD24⁺CD29^hi cells injected per fat pad

Number of positive outgrowths*

Wild-type

Csf1^op/op

60

3/4

0/4

100

7/11

5/11

120

2/4

1/4

200

20/22

12/22

240

3/4

2/4

Repopulating frequency

(95% confidence interval)

1/97

(1/66 to 1/141)

1/261

(1/166 to 1/408)

Limiting dilution analysis of the repopulating frequency of mammary stem cell (MaSC)-enriched cells from either wild-type or Csf1^op/op glands transplanted into wild-type recipients. Cells were injected into the cleared mammary fat pads of three-week-old syngeneic recipients. Results are pooled from four independent experiments (P = 0.00065). *Shown as number of outgrowths per number of injected fat pads. Csf1 = colony stimulating factor 1.

Gyorki et al. Breast Cancer Research 2009 11:R62   doi:10.1186/bcr2353