Additional file 1:

A Quicktime video demonstrating the intraductal transplantation technique.

Format: QT Size: 7.8MB Download file

Additional file 2:

A Word file listing primary antibodies.

Format: DOC Size: 39KB Download file

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Additional file 3:

A Word file containing classification of tumor subtypes by immunoassay. Expression of Her-2, ER, CK-5, and Her-1 by immunostaining may be used to predict tumor subtypes by microarray with a high degree of specificity [9,10]. Based on immunostaining, DCIS.COM generates basal, and SUM-225 and FSK-H7 generate Her-2-overexpressing DCIS-like lesions.

Format: DOC Size: 40KB Download file

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Additional file 4:

A TIF file containing flow-cytometry analysis of DCIS.COM and SUM-225 for co-expression of basal and luminal surface markers. SUM-225 and DCIS.COM were stained by using antibodies to CD44/CD24, CD49f/MUC-1, and CD49f/CD24. The gated subpopulations in each panel were sorted, and the in vivo growth potential was assessed by using intraductal transplantation. As illustrated by FACS-generated dot plots, the majority of cells in DCIS.COM are CD44^hi, CD49f^hi, MUC-1^med, and CD24^med. Therefore, DCIS.COM subpopulations (in Table 1) do not contain CD49f^lo-, CD44^lo-, CD24^hi-, or MUC-1^hi-expressing cells. SUM-225 cells were predominantly CD44^med, CD49f^med, MUC-1^hi, and CD24^hi. Therefore, SUM-225 subpopulations (in Table 1) do not include CD49f^hi-, CD44^hi-, CD24^lo-, and MUC-1^lo-expressing cells.

Format: TIFF Size: 8.2MB Download file

Additional file 5:

A Word file Subgroup comparisons by using Fisher's Exact test.

Format: DOC Size: 115KB Download file

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