Figure 5.

Flow-cytometry analysis of DCIS.COM and SUM-225. Cells grown in 2D were stained by using the indicated anti-human antibodies to the surface markers, CD44/CD24, CD49f/CD24, and CD49f/MUC-1. The antibodies were directly conjugated, except for MUC-1, which was conjugated by using an anti-mouse FITC antibody. The histograms show expression levels for the indicated surface markers. The expression level is arbitrarily designated as low (lo) if the log¹⁰ of median fluorescence intensity (FI) is between about 0 and 2, medium (med) if the FI is between about 2.1 and 3.6, and high (hi) if the FI is higher than about 3.7.