Estrogen receptor action in three dimensions - looping the loop
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* Corresponding author: Jason S Carroll jason.carroll@cancer.org.uk
Cancer Research UK, Cambridge Research Institute, Robinson Way, Cambridge, CB2 0RE, UK
Breast Cancer Research 2010, 12:303 doi:10.1186/bcr2470
Published: 8 February 2010Abstract
Due to advances in genomic technologies, our understanding of estrogen receptor (ER)-mediated transcription in breast cancer cells has evolved significantly in recent years. Genome-wide mapping experiments revealed thousands of ER-binding events, but linking them to the target genes has been an ongoing struggle. A recent paper describes a new technique, called ChIA-PET (chromatin interaction analysis using paired-end tag sequencing), that can directly address these questions. ChIA-PET is an unbiased approach for simultaneously identifying all genome-wide binding events of a transcription factor and those involved in long-range chromatin loops.