Research article
Survival and self-renewing capacity of breast cancer initiating cells during fractionated radiation treatment
-
* Corresponding author: Frank Pajonk fpajonk@mednet.ucla.edu
- † Equal contributors
1 Division of Molecular and Cellular Oncology, Department of Radiation Oncology, David Geffen School of Medicine at University of California, Los Angeles, 10833 Le Conte Avenue, Los Angeles, CA 90095-1714, USA
2 Jonsson Comprehensive Cancer Center, University of California, Los Angeles, 8-684 Factor Building Box 951781, Los Angeles, CA 90095-1781, USA
Breast Cancer Research 2010, 12:R13 doi:10.1186/bcr2479
See related editorial by Nakshatri, http://breast-cancer-research.com/content/12/2/105
Published: 16 February 2010Additional files
Additional file 1:
Figure S1. Control gate of CD24 and CD44 analysis (Figure 1). Fluorescence-activated cell-sorting (FACS) analysis was performed to measure non-specific binding of anti-mouse isotype control PE-conjugated and anti-mouse isotype control APC-conjugated antibodies, and effects of radiation treatment on cell auto-fluorecence.
Format: PDF Size: 203KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 2:
Figure S2. Control gate of ZsGreen-cODC analysis (Figure 4). Cells stably transfected with an empty control vector were irradiated with 5 × 3 Gy (right panel) or sham irradiated (left panel), and cells were analyzed for green auto-fluorescence. Cells were defined as ZsGreen-cODC positive if the fluorescence in the FL-1H channel exceeded the fluorescence level of 99.9% of the empty vector control cells.
Format: PDF Size: 150KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 3:
Figure S3. Control gate of cell membrane PHK26 staining efficiency (Figure 5). After cell membrane staining with PKH26 fluorescence was analyzed in the total population (left panel), non-tumorigenic cells (middle panel), and CICs (right panel). No difference was found for PKH26 efficiency staining between non-tumorigenic cells and CICs.
Format: PDF Size: 89KB Download file
This file can be viewed with: Adobe Acrobat Reader