Table 2

Intracellular concentrations (nmol/g cell weight) of ¹³ C-labeled endogenous metabolites (glycolysis and TCA cycle intermediates, glucose) and lipid metabolites (choline-containing phospholipids, cholesterol)

Control

Lovastatin lactone

Lovastatin

acid

¹³C-lactate

612 ± 36

252 ± 51**

343 ± 15**

glycolysis int

845 ± 21

369 ± 13***

467 ± 52***

TCA cycle int

913 ± 232

189 ± 28**

213 ± 56**

glucose_intracell

1918 ± 382

2691 ± 283*

2758 ± 231*

citrate

323 ± 46

98 ± 33***

153 ± 35**

glutathione

2595 ± 168

871 ± 72***

1149 ± 78**

choline-PL

3757 ± 534

2158 ± 275**

2672 ± 542*

chol C18+C19

3914 ± 582

2125 ± 289**

2467 ± 351**

The values were calculated based on MDAMB468 cell extracts as assessed by ¹H-NMR and ¹³C-NMR. The cells were incubated with 8 μg/mL lovastatin lactone or hydroxy acid for 48 hours. Values are presented as means ± standard deviation of three independent experiments. Significance levels: * P < 0.05; ** P < 0.005; *** P < 0.001 were determined by analysis of variance (with post-hoc pairwise multiple comparison Tukey-test). chol, cholesterol; choline-PL, choline-containing phospholipids; glu, glutamate; glycolysis int, glycolysis intermediates: ¹³C-lactate+¹³C-alanine, TCA int: TCA cycle intermediates: (C2 + C3 + C4)-glutamate + (C2 + C3)-glutamine.

Klawitter et al. Breast Cancer Research 2010 12:R16   doi:10.1186/bcr2485