Figure 8.

Fulvestrant synergizes with NaBu to enhance the DIA sensitivity of MCF-7 cells. (a) Western blot analysis of MYB expression in MCF-7 cells treated with fulvestrant or sodium butyrate (NaBu), alone or in combination, as indicated. (The combination of fulvestrant with 1 mM NaBu is not included because of extensive cell loss due to apoptosis. A ß-tubulin loading control is also shown. (b) MCF-7 cells grown on glass slides were treated with fulvestrant alone or in combination with 0.5 mM or 1 mM NaBu for 72 hours. The cells were then stained with Nile Red (red) for lipid vesicles and 4',6-diamidino-2-phenylindole (DAPI) (blue) for DNA. (c) Apoptosis and (d) lipid accumulation were detected by TUNEL assay and Nile Red staining, respectively, and quantitated by flow cytometry standard deviation is represented by error bars (n = 3).