Additional file 4.

MYB knockdown sensitises ZR-75-1 cells to the differentiation-inducing agent NaBu. (a) ZR-75-1 cells with a doxycycline (Dox)-inducible shRNA targeting MYB [19] were grown on a glass coverslip, treated with or without 5 μg/mL Dox for 24 hours before exposure to the indicated concentrations of sodium butyrate (NaBu) for three days. They were then stained with Nile Red (red) for lipid vesicles and DAPI (blue) for DNA detection. (b) Flow cytometric analysis of TUNEL staining for apoptotic cells. ZR-75-1 cells stably transfected with inducible shRNA treated for 24 hours with or without Dox, were grown in the presence or absence of 1 mM NaBu for a further 24 hours, and were then assayed for apoptosis using TUNEL. Standard deviation is represented by error bars.

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Drabsch et al. Breast Cancer Research 2010 12:R55   doi:10.1186/bcr2614