Figure 1.

Twist and vimentin expression in HeLa cells spiked in blood of normal volunteers. ARIOL system images of HeLa cells spiked in the blood of normal volunteers. (A) Row I: Positive control for Twist. HeLa cells were stained with pan-CK A45-B/B3 antibody/secondary FITC anti-mouse antibody (green)/Twist anti-rabbit antibody/Alexa Fluor 555 anti-rabbit antibody (orange). Row II: Negative control for Twist. Cells were stained with pan-CK A45-B/B3 antibody/FITC anti-mouse (green) and Alexa Fluor 555 IgG isotype antibody. Cell nuclei were stained with DAPI (blue). Original magnification, x400. (B) Row I: Positive control for vimentin. Cells were stained with pan-CK A45-B/B3 antibody/FITC anti-mouse antibody (green)/vimentin anti-rabbit antibody/Alexa Fluor 555 anti-rabbit antibody (orange). Row II: Negative control for vimentin. Cells were stained with pan-CK A45-B/B3 antibody/FITC anti-mouse antibody (green) and Alexa Fluor 555 IgG isotype antibody. Row III: Negative control for pan-CK. Cells were stained with FITC IgG isotype antibody/vimentin anti-rabbit antibody/Alexa Fluor 555 anti-rabbit antibody (orange). Cell nuclei were stained with DAPI (blue). Original magnification, x400. ARIOL system = automated image analysis system; CK = cytokeratin; FITC = fluorescein isothiocyanate; HeLa = patient with cervical adenocarcinoma from which the cell line was derived; IgG = immunoglobulin G; DAPI = 4',6-diamidino-2-phenylindole.

Kallergi et al. Breast Cancer Research 2011 13:R59   doi:10.1186/bcr2896
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