Additional file 1.

Triple-staining control experiments in HeLa cells. Triple-staining control experiments in HeLa cells analysed using a confocal laser-scanning microscope. (I) Representative confocal laser-scanning photomicrographs of positive controls for Twist anti-mouse antibody. HeLa cells were stained with pan-CK A45-B/B3 antibody/Zenon Alexa Fluor 488 (green)/Twist anti-mouse/Alexa Fluor 633 anti-mouse IgG (blue)/vimentin anti-rabbit antibody/Alexa Fluor 555 anti-rabbit antibody (red). Original magnification, x500. (II) Negative control for Twist. HeLa cells stained with pan-CK A45-B/B3 antibody/Zenon Alexa Fluor 488 (green)/Alexa Fluor 633 anti-mouse IgG (blue)/vimentin anti-rabbit antibody/Alexa Fluor 555 anti-rabbit antibody (red). Original magnification, x500. (II) Negative control for vimentin. HeLa cells were stained with pan-CK A45-B/B3 antibody/Zenon Alexa Fluor 488 (green)/Twist anti-mouse antibody/Alexa Fluor 633 anti-mouse antibody (blue)/Alexa Fluor 555 IgG isotype antibody (red). Original magnification, x500. CK = cytokeratin; HeLa = patient with cervical adenocarcinoma, from which the cell line was derived; IgG, immunoglobulin G.

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Kallergi et al. Breast Cancer Research 2011 13:R59   doi:10.1186/bcr2896