Additional file 2.

Twist and vimentin expression in CTCs of breast cancer patients. Representative confocal laser-scanning microscopic images of Twist-or vimentin-expressing CTCs of breast cancer patients. (I) Representative confocal laser-scanning microscopic images of breast cancer patients' cytospin double-stained with monoclonal pan-CK (A45-B/B3)/Alexa Fluor 488 anti-mouse antibody (green)/polyclonal Twist anti-rabbit antibody and Alexa Fluor 555 anti-rabbit antibody (red). Original magnification, x600. (II) Representative images of cytospin double-stained with monoclonal pan-CK (A45-B/B3)/Alexa Fluor 488 anti-mouse antibody (green)/polyclonal vimentin anti-rabbit antibody and Alexa Fluor 555 anti-rabbit antibody (red). Original magnification, x600. CK = cytokeratin; CTCs = circulating tumour cells; FITC = fluorescein isothiocyanate.

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Kallergi et al. Breast Cancer Research 2011 13:R59   doi:10.1186/bcr2896