Table 2

Effect of prolonged consumption of a high-fat diet on the levels of various cytokines in the sera of BALB/c mice injected with 4T1 cells

Control diet

High-fat diet


C5a (ng/ml)

22.0 ± 2.7

29.0 ± 3.1a

sICAM-1 (ng/ml)

494.2 ± 22.6

651.5 ± 29.4a

IL-16 (ng/ml)

150.8 ± 8.0

190.0 ± 8.4a

M-CSF (pg/ml)

714.1 ± 29.3

1,149.5 ± 35.6a

TIMP-1 (ng/ml)

4.4 ± 0.4

6.2 ± 0.4a

TREM-1 (pg/ml)

684.7 ± 17.8

967.8 ± 21.6a

Leptin (ng/ml)

138.0 ± 0.04

187.8 ± 11.3a


Mice were fed on the high-fat (60% kcal) or control diet (10% kcal) for 16 weeks and injected with 4T1 cells, as described in Materials and Methods. At 25 days after the 4T1 cells injection, blood samples were collected from the mice, and the sera were prepared. The serum levels of complement fragment 5a (C5a), soluble intercellular adhesion molecule (sICAM)-1, interleukin (IL)-16, macrophage colony-stimulating factor (M-CSF), tissue inhibitor of matrix metalloproteinase (TIMP)-1, triggering receptor expressed on myeloid cells (TREM)-1, and leptin were measured with the appropriate ELISA kits. Values represent the mean ± SEM (n = 30). aSignificantly different from the control group, P < 0.05.

Kim et al. Breast Cancer Research 2011 13:R78   doi:10.1186/bcr2927

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