Generation of OCT4-transduced breast cells (OTBCs) from primary cultures of mammary cells. Graphical representation of the method used to generate OTBCs from normal breast tissue. Representative images of control cells (empty lentiviral vector) and OTBCs are shown 2 weeks after transduction of OCT4 and seeding in feeder cultures of mouse embryonic fibroblasts in human embryonic stem cell (hESC) media. The OTBCs-86L1 image is a representative example of cells (passage 25) grown in suspension in mammosphere media after cell expansion.
Beltran et al. Breast Cancer Research 2011 13:R94 doi:10.1186/bcr3019