Figure 9.

TWIST1 is downstream of ARTEMIN (ARTN) stimulated Protein kinase B (AKT) activation in estrogen receptor (ER) negative mammary carcinoma (ER-MC). (a) Western blot analyses for ARTN, TWIST1, phospho AKT (pAKT-Ser473) and AKT in BT549 cells with forced expression of TWIST1 ± siARTN. β-ACTIN was used as loading control for cell lysates. The sizes of detected protein bands in kiloDalton (kDa) are shown on the right. (b) Colony formation in soft agar. (c) Cell growth (number) in 3D matrigel culture. (d) Cell migration, and (e) Cell invasion was determined for BT549-TWIST1 cells ± siARTN. siRNA control-treated vector (VEC) cells are presented as 100%. **, P < 0.01; ***, P < 0.001.