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Correction

Correction: A p53-independent role of Mdm2 in estrogen-mediated activation of breast cancer cell proliferation

Angelika Brekman, Kathryn E Singh, Alla Polotskaia, Nandini Kundu and Jill Bargonetti*

Author Affiliations

Department of Biological Sciences, Hunter College and The Graduate Center Biochemistry and Biology Programs, CUNY, 695 Park Ave, New York, NY 10065, USA

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Breast Cancer Research 2012, 14:302  doi:10.1186/bcr3130

The electronic version of this article is the complete one and can be found online at: http://breast-cancer-research.com/content/14/2/302


Published:29 February 2012

© 2012 BioMed Central Ltd

Correction

Following publication of our article [1], the authors noticed that the data incorporated into Figure 4f (1) was incorrect. The first column should be changed from 44, 13 and 46 to read 43, 13 and 44 instead. A corrected version of Figure 4f (1) can be found overleaf.

thumbnailFigure 4. Mdm2 knockdown in estrogen-treated MCF-7 cells inhibits cell proliferation. Clonal MCF-7 cell lines with mdm2 shRNA or control vector were treated with 2 μg/ml doxycycline (DOX) for three days to induce shRNA expression, followed by 10 nM estrogen (E2) for five days in the presence of DOX. (a) Western blot analysis of Mdm2, p53, p21 and Actin protein levels from whole cell lysates. (b) Number of cells was determined by Guava Viacount assay. 10,000 cells were seeded at beginning of treatments. (c) Fluorescence activated cell sorting (FACS). Cells were harvested, fixed in 30% ethanol, and cellular DNA was stained with propidium iodide. (d) Western blot analysis of Bcl-2 and Actin protein levels from whole cell lysates. (e) MCF-7 cells, grown in matrigel for three weeks, formed mass structures of three sizes: large, intermediate and small. (f) MCF-7 cells, grown in matrigel for three weeks in the presence of 10 nM estrogen and in the absence or presence of 2 μg/ml doxycycline, were fixed and stained with propidium iodide. Masses of different sizes (large, intermediate and small) were counted and presented as percent of the total population. On average, a total of 300 masses were counted. Averages of three independent experiments are shown. The significance in the percent change of large and small masses was determined by the Student t-test (P < 0.05).

Competing interests

The authors declare that they have no competing interests.

References

  1. Brekman A, Singh KE, Polotskaia A, Kundu N, Bargonetti J: A p53-independent role of Mdm2 in estrogen-mediated activation of breast cancer cell proliferation.

    Breast Cancer Research 2011, 13:R3. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL