Table 1

Pigment epithelium-derived factor expression in normal versus breast tumor tissue samples

Tissue sample

n

PEDF status

Intensity score


Recurrence tumors

28 (47.6%)a

Positive

> 150

31 (52.4%)b

Negative

≤ 25

Primary tumors

40 (67.8%)a

Positive

> 150

19 (32.2%)b

Negative

≤ 25

Endocrine-responsive tumors

125 (83.3%)c

Positive

> 150

25 (16.7%)d

Negative

≤ 25

Normal tissue

5 (100%)

Positive

≥ 200

0 (0%)

Negative

≤ 25


A total of 209 breast cancer patients were initially treated with tamoxifen and 59 patients developed recurrence disease after a mean follow-up of 93 months. Immunohistochemistry (IHC) staining was performed on tissue microarrays (TMAs) constructed from recurrence breast tumors (n = 59) and matched primary breast tumors (n = 59). Normal background breast tissue was also used for comparison. TMAs were also constructed from endocrine-responsive tumor tissues (n = 150). A semi-automated quantitative image analysis system (ACIS II) was used to quantitate the staining of the TMA slides. For IHC analysis, pigment epithelium-derived factor (PEDF) staining was quantified as an intensity score (scale 0 to 255). aP < 0.00003, number of PEDF-positive recurrence tumors versus PEDF-positive primary tumors. bP < 0.000001, number of PEDF-negative recurrence tumors versus PEDF-negative primary tumors. cP < 0.00008, number of PEDF-positive recurrence tumors versus PEDF-positive endocrine-responsive tumors. dP < 0.000001, number of PEDF-negative recurrence tumors versus PEDF-negative endocrine-responsive tumors.

Jan et al. Breast Cancer Research 2012 14:R146   doi:10.1186/bcr3356

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