Table 3 |
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|
Comparative analysis of ER by immunohistochemistry (ER1D5), DCC ([3H]oestradiol-binding) and hydroxylapatite ([3H]oestradiol-binding and EI) |
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|
Tissular ER |
Cytosolic ER |
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|
|
|
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|
IHC |
DCC |
Hydroxylapatite |
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|
|
|
|
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|
Case no. |
IS |
n (fmol/mg protein) |
n (fmol/mg protein)* |
EI (%)† |
|
|
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|
1 |
10 |
39 |
27 (20 + 7) |
74.5 |
|
2 |
20 |
69 |
62 (54 + 8) |
86.8 |
|
3 |
20 |
32 |
67 (21 + 46) |
31.5 |
|
4 |
40 |
38 |
35 (12 + 23) |
34.4 |
|
5 |
130 |
23 |
130 (91 + 39) |
70.0 |
|
6 |
130 |
83 |
151 (62 + 89) |
41.1 |
|
7 |
160 |
163 |
166 (30 + 136) |
18.0 |
|
8 |
160-200 |
257 |
209 (58 + 151) |
27.5 |
|
9 |
260 |
23 |
103 (64 + 39) |
62.1 |
|
10 |
360 |
265 |
230 (114 + 116) |
49.6 |
|
11 |
360-400 |
400 |
360 (190 + 170) |
52.6 |
|
12 |
360-400 |
354 |
337 (82 + 255) |
24.5 |
|
13 |
360-400 |
178 |
193 (60 + 133) |
31.0 |
|
14 |
360-400 |
92 |
174 (36 + 138) |
20.8 |
|
15 |
360-400 |
312 |
390 (156 + 234) |
40.0 |
|
|
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|
*Hydoxylapatite corresponds specifically to [3H]oestradiol extracted successively from hydroxylapatite with KCl and ethanol (ie 27 = 20 + 7). †EI = ([3H] oestradiol) [KCl] × 100/([3H]oestradiol) [KCl] + ([3H]oestradiol) [EtOH]. IHC, immunohistochemistry; IS, immunostaining score. |
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|
Maaroufi et al. Breast Cancer Res 2000 2:444 doi:10.1186/bcr92 |
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