Table 1

PCR and DHPLC conditions for the mutational analysis of E-cadherin

Annealing
 Amplicon
 DHPLC

temperature
 length
 temperature
Exon no
 (°C)
 (bp)
 (°C)
1
 70
 285
 66
2
 55
 377
 62
3
 60
 352
 58, 61
4
 60
 351
 60
5
 60
 398
 58
6
 58
 246
 59
7
 60
 329
 60, 62, 64
8
 58
 225
 59, 62
9
 55
 252
 59
10
 55
 309
 58
11
 60
 243
 61
12
 60
 326
 59, 61
13
 58
 297
 58
14
 60
 206
 61
15
 60
 244
 58
16
 60
 318
 59

Salahshor et al. Breast Cancer Res 2001 3:199   doi:10.1186/bcr295

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