Breast Cancer Res
Volume 5
Suppl 1 |
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Oral presentationGenetic manipulation of the mammary gland by transplantationP Edwards Hutchison-MRC Research Centre, University of Cambridge, Cambridge, UK 24 th Congress of the International Association for Breast Cancer Research. Advances in human breast cancer research: preclinical models Sacramento, USA. 1-5 November 2003 Breast Cancer Res 2003,
5(Suppl 1):6doi:10.1186/bcr665
| Published: |
31 October 2003 |
Oral presentationMammary epithelium can be reconstituted in vivo by transplanting fragments of mammary epithelium, or suspensions of mammary epithelial cells, into the 'cleared' mammary fat pad of a syngeneic recipient mouse. A 'cleared' mammary fat pad is one from which the natural epithelium has been removed at 3 weeks of age. Genes can be introduced into the epithelium before transplantation using retrovirus vectors, or the epithelium can be taken from a knockout mouse [1]. The applications of transplantation, and its advantages and disadvantages compared with transgenesis will be surveyed, including the ability to use hormone-insensitive promoters; to introduce genes into clones of cells rather than whole tissues; the ease of studying early preneoplastic change; and the use of transplantation with transgenic knockouts, to rescue embryonic lethals and to distinguish systemic from local effects [2]. References
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Dev Biol 1999, 212:1-11. PubMed Abstract | Publisher Full Text
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