|
Evaluation of Her-2/neu gene status using chromogenic in situ hybridization |
|
| Amplification |
Her-2/neu gene status |
|
|
|
| High-level |
>10 copies or large cluster of amplicon per nucleus in >50% of cancer cells |
| Low-level |
6–10 copies or small cluster of amplicon per nucleus in >50% of cancer cellsa |
| None |
1–5 copies per nucleus of cancer cellsb |
|
aVerify gene amplification or chromosome 17 aneuploidy with SPOT-Light Chromosome 17 Centromeric Probe. bIf 3–5 copies per nucleus, verify chromosomal aneuploidy with SPOT-Light Chromosome 17 Centromeric Probe. | |
Madrid and Lo Breast Cancer Res 2004 6:R593 doi:10.1186/bcr915 |
|