Figure 2.

Cyclo-oxygenase (COX) muscarinic receptors in TMps. Upper panel: macrophages from 7-day LMM3 tumor-bearing mice (TMps) were treated with carbachol (CARB) (100 nM) in the absence or presence of 1 μM indomethacin (INDO) 10 μM NS-398, 1 μM atropine (AT) or 4-DAMP. COX activity was measured by prostaglandin E₂ (PGE₂) liberation by TMps as pg/10⁶ cells. Values are means ± SEM for three experiments. *P < 0.001 compared with basal; ^#P < 0.001 compared with CARB; NS, not significantly different from basal by Tukey's modified t-test. Lower panel: Western blot assay to detect COX isoforms and muscarinic receptors (M) in lysates of TMps. Molecular masses of the bands indicated on the left are coincident with COX-1, COX-2 and M₃ proteins. One representative experiment of three is shown.