Table 2

Ex vivo detection of metastasis after intracardiac injection (nude mice)


H2LN (n = 8)
D3H1 (n = 5)

Experimental end-point
Week 5
Week 10
Skeletal metastasis
8/8
4/5
          Spine
88%a
60%a
          Skull
75%a
40%a
          Scapula
75%
40%a
          Femur/tibia
63%a
0%a, b
          Clavicle
50%
0%
          Upper/lower jaw
13–23% ND
0–20%
Ribs and associated tissues
8/8a
4/5a
Brain
7/8a
2/5a
Lungs
6/8a
2/5a
Lymph nodes (axillary/brachial)
5/8a
2/5
Other soft tissues (heart, liver, pancreas, kidney, adrenals, uterus, salivary gland)
4/8
2/5

aMetastasis confirmed by histology in at least one animal sample. bThree out of five mice exhibited in vivo leg signal, and metastasis was confirmed by histology. ND, tissue not analyzed by histology.

Jenkins et al. Breast Cancer Research 2005 7:R444   doi:10.1186/bcr1026