Figure 2.

Promoting activity of newly designed forms of pNeu in various cell lines differentially expressing c-erbB-2 (a) Schematic representation of various forms of pNeu constructed upstream of the murine α1,3galactosyltransferase (mαGalT) cDNA. The transcription start site is indicated by arrows, and the 5' end points of the various forms of pNeu by bold bars. (b) Northern blot analysis of expression of mαGalT driven by the CMV promoter (pCMV) and the various forms of constructed pNeu. (c) Northern blot analysis of c-erbB-2 expression in stably transfected HEK-293, MCF-7 and SK-BR-3 cells. Total RNAs were electrophoresed and probed with [α-³²P]-random-labeled mαGalT or c-erbB-2 cDNA, and thereafter with glyceraldehyde 3-phosphate-dehydrogenase (GAPDH) cDNA as a control.