Figure 5.

Effect of propofol-docosahexaenoic acid (propofol-DHA) and propofol-eicosapentaenoic acid (propofol-EPA) conjugates on cell migration. MDA-MB-231 cells (10⁴) were incubated for 4 hours with 25 μM DHA (D), 25 μM EPA (E), 25 μM propofol (P), 25 μM each of D+P, 25 μM each of E+P, and 25 μM propofol-DHA (D-P) or 25 μM propofol-EPA (E-P) using transwell plates. The control cells (C) were treated with equal amounts of ethanol. Cells that migrated through the filter were counted under a microscope as described in the text. Results are means ± SEM for four experiments. The results were analyzed by analysis of variance and Dunnett's multiple comparison test to control the Type I experimental wise error. Significant differences from the control (P < 0.05) are indicated with an asterisk.