Figure 6.

Western blot analysis of thymidylate synthase and ternary complex. (a) Untreated cells; (b) 5-FU (1 μg/ml); (c) Dox (0.1 μg/ml)→Pacl (0.1 μg/ml); (d) Dox (0.1 μg/ml)→Pacl (0.1 μg/ml)→48-h washout→5-FU (1 μg/ml). 50 μg of protein were loaded for the controls and treated samples.