Figure 3.

Peptide mass fingerprinting of in-gel tryptic digest of the 90 kDa band generated with a matrix-assisted laser desorption ionization-time-of-flight mass spectrometer. Protein bands indicated by the arrowhead in Fig. 1 were subjected to in-gel digestion and analyzed by mass spectrometry. The tryptic peptides from this band showed the presence of six peptides corresponding to heat shock protein 90 (Hsp90) as one of the proteins that were found in the altered protein-protein interaction pattern of p53 with and without PRIMA-1 treatment.