Figure 4.

Induction of G₁ arrest in MCF-7 cells treated with rapamycin plus CN-A. Cells were cultured (a) without or (b) with 10 μg/ml CN-A, (c) 0.5 ng/ml rapamycin, or (d) 0.5 ng/ml rapamycin plus 10 μg/ml CN-A for 6 days, and DNA histograms were then analyzed. CN-A, cotylenin A.