Table 4 |
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|
Array-based comparative genomic hybridization validation by DNA quantitative PCR of ERBB2 |
||||||
|
Tumor ID |
DNA |
RNA |
Protein |
|||
|
|
||||||
|
aCGH RP11-552K3 |
Light cycler |
TaqMan |
Hercept |
|||
|
|
||||||
|
Cy3 |
Cy5 |
Relative ratio |
Relative alleles |
Ratio |
Staining |
|
|
|
||||||
|
40 |
1.05 |
1.13 |
0.67 |
1 |
0.51 |
- |
|
71 |
1.28 |
0.58 |
0.94 |
2 |
2.01 |
- |
|
88 |
1.48 |
0.45 |
1.08 |
2 |
2.08 |
- |
|
268 |
1.51 |
0.76 |
0.94 |
2 |
1.78 |
- |
|
313 |
0.99 |
1 |
0.82 |
2 |
0.95 |
- |
|
348 |
1.06 |
0.8 |
0.88 |
2 |
0.99 |
- |
|
352 |
1.64 |
0.76 |
0.88 |
2 |
n.d.* |
- |
|
476 |
1.03 |
0.88 |
0.94 |
2 |
1.72 |
- |
|
493 |
1.25 |
0.97 |
0.88 |
2 |
n.d. |
- |
|
277 |
1.14 |
0.99 |
0.94 |
2 |
0.67 |
2+ |
|
445 |
1.42 |
0.87 |
0.88 |
2 |
0.43 |
2+ |
|
316 |
2.41 |
0.43 |
1.51 |
3 |
5.61 |
3+ |
|
369 |
2.45 |
0.29 |
3.88 |
8 |
16.1 |
3+ |
|
|
||||||
|
ERBB2 validation of array-based comparative genomic hybridization (aCGH) DNA copy number using LightCycler Her2/Neu DNA quantification kit (Roche), TaqMan Assay on Demand Hs. 00170433_m1 (Applied Biosystems), and protein quantification (Herceptest®). * n.d.: Not done |
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|
Naylor et al. Breast Cancer Research 2005 7:R1186 doi:10.1186/bcr1356 |
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