Figure 3.

Proliferative response of MCF-7 cells overexpressing different SULT1A1 allozymes. MCF-7 cells overexpressing SULT1A1*2 proliferate faster in response to 17β-estradiol (E2) than cells overexpressing SULT1A1*1 (P = 0.002). Cells stably expressing equal levels of SULT1A1 mRNA (SULT1A1*1 or SULT1A1*2) were selected for study. Cells were plated onto 96-well plates and, in triplicate, treated with increasing concentrations of E2 (0, 0.1, 1 and 100 nM) in charcoal-stripped media. Data were corrected for proliferation in the absence of E2 to calculate the proliferative index. Data for 'pCR3.1' cells were generated in a MCF-7 control cell line mock-transfected with the empty pCR3.1 vector.