Figure 1.

Differential CD24 expression distinguishes between mammary gland subtypes. (a) Cells were sorted on the basis of forward scatter and side scatter. (b) Dead cells (DAPI bright) were excluded. (c) B lymphocytes and immature T lymphocytes are CD24⁺ and, therefore, CD45 staining was used to exclude all leukocytes. (d) Doublets and higher order cell clumps were excluded using a time-of-flight approach (boxed cells represent single cells). (e) Typical CD24 staining profile, indicating the three populations of cells: CD24^High (69.9 ± 5.5%; n = 15), CD24^Low (22.2 ± 5.6%; n = 15) and CD24^Negative (6.3 ± 1.5%; n = 15). DAPI = 4',6-diamidino-2-phenylindole dihydrochloride; FITC, fluorescein isothiocyanate; PE-Cy5, phycoerythrin-Cy5.